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Electrochemical Aptasensor Based on Polycarboxylic Macrocycle Modified with Neutral Red for Aflatoxin B1 Detection
Author(s) -
Evtugyn Gennady,
Porfireva Anna,
Stepanova Veronika,
Sitdikov Rusal,
Stoikov Ivan,
Nikolelis Dimitrios,
Hianik Tibor
Publication year - 2014
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400328
Subject(s) - detection limit , aptamer , electrochemistry , chemistry , analyte , glassy carbon , aflatoxin , electrode , electron transfer , covalent bond , combinatorial chemistry , chromatography , photochemistry , organic chemistry , cyclic voltammetry , food science , genetics , biology
Novel electrochemical aptasensors based on glassy carbon electrodes modified with electropolymerized Neutral red and polycarboxylated macrocyclic ligands onto which the DNA aptamers were covalently attached have been developed for detection of Aflatoxin B1 (AFB1). The interaction with an analyte resulted in the decrease of the cathodic peak current of the probe measured by CV and in the increase of the electron transfer resistance determined by EIS. The limit of detection was found to be 0.1 nM for CV and 0.05 nM for EIS methods, respectively. The aptasensor makes it possible to detect AFB1 in peanuts, cashew nuts, white wine and soy sauce with a recovery of 85–100 %.