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Direct Determination of miR‐21 in Total RNA Extracted from Breast Cancer Samples Using Magnetosensing Platforms and the p19 Viral Protein as Detector Bioreceptor
Author(s) -
TorrenteRodríguez R. M.,
Campuzano S.,
LópezHernández E.,
Granados R.,
SánchezPuelles J. M.,
Pingarrón J. M.
Publication year - 2014
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400317
Subject(s) - detection limit , rna , detector , breast cancer , rna extraction , streptavidin , microbiology and biotechnology , chemistry , cancer , medicine , chromatography , biotin , biology , gene , biochemistry , physics , optics
A novel magnetobiosensing approach for the rapid, sensitive and selective miR‐21 detection is reported involving the use of a specific RNA probe (antimiR‐21), streptavidin‐magnetic beads (Strep‐MBs), the siRNA Binding Protein p19 as detector bioreceptor, and amperometric detection with the H 2 O 2 /hydroquinone (HQ) system at disposable screen‐printed carbon electrodes. The magnetosensor exhibited a dynamic range from 1.4 to 10 nM and a detection limit of 4.2 fmol of synthetic target miR‐21 without any amplification step in 75 min. The usefulness of the approach was evaluated by analyzing total RNA (RNA t ) extracted from metastatic breast cancer cell lines, human tissues and breast cytologies.