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A Highly Sensitive and Selective Enzymatic Biosensor Based on Direct Electrochemistry of Hemoglobin at Zinc Oxide Nanoparticles Modified Activated Screen Printed Carbon Electrode
Author(s) -
Palanisamy Selvakumar,
Karuppiah Chelladurai,
Chen ShenMing,
Periakaruppan Prakash
Publication year - 2014
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400304
Subject(s) - biosensor , zinc , chemistry , hydrogen peroxide , electrochemistry , detection limit , nitrite , nuclear chemistry , electrode , carbon paste electrode , inorganic chemistry , redox , analytical chemistry (journal) , cyclic voltammetry , chromatography , organic chemistry , nitrate , biochemistry
A sensitive enzymatic biosensor has been developed for the detection of hydrogen peroxide (H 2 O 2 ), nitrite ( ${{\rm NO}{{- \hfill \atop 2\hfill}}}$ ) and trichloroacetic acid (TCA) by using hemoglobin (Hb) immobilized on activated screen printed carbon electrode (ASPCE) and zinc oxide (ZnO) composite. A pair of well defined redox peaks is observed with a heterogeneous electron transfer rate constant (K s ) of 5.27 s −1 for Hb at ASPCE/ZnO. The biosensor exhibits the detection of H 2 O 2 , TCA and ${{\rm NO}{{- \hfill \atop 2\hfill}}}$ in the concentration range of 0.5–129.5 µmol L −1 , 2.5–72.5 mmol L −1 and 0.2–674 µmol L −1 with the detection limit of 0.083 µmol L −1 , 0.12 mmol L −1 and 0.069 µmol L −1 , respectively.