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Dynamic Structures of DNA Heptamers with Different Central Trinucleotide Sequences Studied by Electrochemical and Spectral Methods
Author(s) -
Pilarova Iveta,
Kejnovska Iva,
Vorlickova Michaela,
Trnkova Libuse
Publication year - 2014
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400288
Subject(s) - supramolecular chemistry , dna , cyclic voltammetry , electrochemistry , duplex (building) , chemistry , molecule , g quadruplex , crystallography , combinatorial chemistry , analytical chemistry (journal) , stereochemistry , chromatography , electrode , organic chemistry , biochemistry
A short, single‐stranded DNA fragment d(GCGAAGC) and its analogs d(GC NNN GC) carrying different trinucleotide sequences in the center of the molecule (AAA,CCC, GGG or TTT) were investigated by spectral (CD and UV spectra) and electrochemical methods (voltammetry, polyacrylamide gel electrophoresis). Our results show that (i) the central triplet GAA or AAA dramatically stabilizes DNA hairpins, (ii) a stem‐loop configuration is not supported by CCC or TTT sequences, instead, these heptamers adopt bimolecular duplex forms. Finally (iii), in the case of GGG sequence a very stable supramolecular G‐quadruplex is formed.