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Electrochemical Biosensors Based on Enzymatic Reactor of Silver Solid Amalgam Powder for Measurements in Flow Systems
Author(s) -
Josypčuk Oksana,
Barek Jiří,
Josypčuk Bohdan
Publication year - 2014
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400224
Subject(s) - biosensor , ascorbic acid , tyrosinase , glucose oxidase , amalgam (chemistry) , detection limit , chemistry , immobilized enzyme , laccase , flow injection analysis , chromatography , catalase , enzyme , electrode , biochemistry , food science
An enzymatic reactor based on silver solid amalgam powder was suggested as the main part of biosensors in flow systems for the first time. Biosensors were tested with following enzymes: ascorbate oxidase, glucose oxidase, catalase, tyrosinase and laccase. The current response of each biosensor was optimized with respect to the detection potential, flow rate, the injection and reactor volume. Relative standard deviation for detection with the studied enzymes was found to be in the range of 0.81–2.1 %. The biosensor with the ascorbate oxidase reactor was used for determination of ascorbic acid in the vitamin tablets Celaskon.