Premium
Flow Injection Analysis System Using Magnetic Beads, Screen Printed Electrodes and Magnets
Author(s) -
Biscay Julien,
Begoña González García María,
García Agustín Costa
Publication year - 2014
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400212
Subject(s) - magnet , electrode , flow injection analysis , materials science , streptavidin , biotin , sensitivity (control systems) , linear range , analytical chemistry (journal) , flow (mathematics) , chromatography , chemistry , detection limit , mechanical engineering , electronic engineering , physics , mechanics , biochemistry , engineering
Here is presented a Flow Injection Analysis (FIA) system using a flow cell with an integrated magnet, applied to biotin determination. The mixture of magnetic beads modified with streptavidin (Strep‐MB), biotin and B‐HRP is left 15 minutes under stirring and then a washing step is performed in an automatic way thanks to the external magnets coupled in the FIA system. After the immobilization of the MBs on the surface of the electrode, 3,3′,5,5′‐Tetramethylbenzidine (TMB) is injected. The linear range obtained is between 0.5 to 10 pM of biotin and the sensitivity is 85 nA/pM.