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A Direct Electrochemical Detection Method of Melanoma Based on Melanoma Biomarker
Author(s) -
Mossberg Michal,
Vernick Sefi,
Ortenberg Rona,
Markel Gal,
Diamand Yosi Shacham,
Rishpon Judith
Publication year - 2014
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400150
Subject(s) - biochip , biosensor , melanoma , amperometry , biomarker , tyrosinase , chip , computer science , chemistry , materials science , nanotechnology , electrode , cancer research , electrochemistry , medicine , enzyme , biochemistry , telecommunications
A new method of detecting and diagnosing melanoma based on melanoma biomarker was developed and its feasibility demonstrated. The method is based on an electrochemical biosensor platform comprised of a special biochip and device, performing a multi‐channel amperometric detection of the enzymatic activity of tyrosinase, an enzyme biomarker of melanoma. The newly developed biosensor platform is able to electrochemically detect tyrosinase activity in fresh biopsy samples. This bioelectrochemical detection method is rapid, yielding results within minutes from biopsy removal. Using “as is” biopsy samples, without pretreatment, simplifies the process, saves time and reduces cost and labor dramatically. Using modern portable microelectronics provides an accurate biomarker expression measurement at the “point of care” increasing the accessibility of new bio‐chip technologies to the public.