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Detection of α‐Methylacyl‐CoA Racemase in Serum and Urine Using a Highly Sensitive Electrochemical Immunodetector
Author(s) -
Wang Jiapeng,
Yau SiuTung
Publication year - 2014
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400123
Subject(s) - detection limit , electrode , urine , chromatography , chemistry , gating , electrochemistry , cancer detection , cancer , medicine , biochemistry , physiology
The detection of α‐methylacyl‐CoA racemase (AMACR), a novel biomarker for prostate cancer, is demonstrated in serum and urine using a novel immuno‐detection method. The detection system consists of a three‐electrode conventional electrochemical cell modified with a gating electrode for applying a gating voltage V G to the immune complex immobilized on the working electrode to provide signal amplification. The detection system is realized by integrating gating electrodes with screen‐printed electrodes. This detection method does not require involved sample preparation procedures. The detection was demonstrated in serum and urine samples on the nanogram/mL level with V G equal to 0.6 V. Detection in serum was also performed on the picogram/mL level with a limit of 100 picogram/mL with V G =0.6 V being a necessary condition.