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Simultaneous Detection of Homocysteine and Cysteine in the Presence of Ascorbic Acid and Glutathione Using a Nanocarbon Modified Electrode
Author(s) -
Lee P. T.,
Lowinsohn D.,
Compton R. G.
Publication year - 2014
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400091
Subject(s) - ascorbic acid , cysteine , chemistry , glutathione , homocysteine , catechol , detection limit , electrochemistry , selectivity , biosensor , electrode , nuclear chemistry , inorganic chemistry , chromatography , organic chemistry , biochemistry , catalysis , enzyme , food science
Abstract The simultaneous electrochemical detection of homocysteine and cysteine using an absorbed ortho ‐quinone species, catechol, at the nanocarbon modified glassy carbon electrode was achieved via 1,4‐Michael addition reaction. The detection was done in the presence and the absence of each other as well as with both glutathione and ascorbic acid present in order to investigate the selectivity of homocysteine and cysteine. A determination of homocysteine sensitivity is (0.882±0.296) nA nM −1 with a LOD of ca. 11 nM and cysteine sensitivity is (7.501±0.202) mA µM −1 with a LOD of ca. 5.0 µM within a range of 0–0.1 mM.