Premium
Development of TiO 2 and Au Nanocomposite Electrode as CEA Immunosensor Transducer
Author(s) -
Aslan Sema,
Anik Ülkü
Publication year - 2014
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400086
Subject(s) - detection limit , electrode , materials science , analyte , nanocomposite , nanoparticle , carbon paste electrode , transducer , colloidal gold , analytical chemistry (journal) , chromatography , nanotechnology , chemistry , electrochemistry , cyclic voltammetry , physics , quantum mechanics
A carbon paste electrode (CPE) was modified by titanium(IV) oxide microparticles and then gold nanoparticles were dispersed in chitosan and immobilized on the CPE surface. As result a nanostructure modified composite electrode was obtained. Carcinoembriyonic antigen (CEA) was chosen as model analyte and the developed electrode was used as CEA immunosensor transducer for the first time. Two linear ranges of 0.01–1 ng/mL and 1–20 ng/mL with corresponding correlation coefficients R 2 =0.986 and R 2 =0.990, were found respectively. The limit of detection of the developed immunosensor was calculated as 0.01 ng/mL with 2.8 % relative standard deviation ( n =5). The developed system was applied for CEA detection in synthetically prepared serum sample and very promising recovery values were obtained.