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Electroactive Prussian Blue Encapsulated Iron Oxide Nanostructures for Mediator‐Free Cholesterol Estimation
Author(s) -
Sharma Rachna,
Sinha R. K.,
Agrawal Ved Varun
Publication year - 2014
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400050
Subject(s) - prussian blue , biosensor , cholesterol oxidase , materials science , indium tin oxide , electron transfer , nanostructure , oxide , nanoparticle , substrate (aquarium) , selectivity , nanometre , glucose oxidase , analytical chemistry (journal) , electrochemistry , chemical engineering , nanotechnology , chemistry , electrode , cholesterol , thin film , photochemistry , chromatography , organic chemistry , metallurgy , oceanography , engineering , biochemistry , catalysis , geology , composite material
Iron oxide nanoparticles of size ∼10 nm have been encapsulated into four nanometer thick shells of Prussian blue and were then electrophoretically deposited onto an indium tin oxide substrate. The immobilization of cholesterol oxidase has been done onto the nanostructured film to investigate the kinetic parameters and biosensing characteristics. The fabricated bioelectrode exhibits an electron transfer coefficient and a charge transfer rate constant of 0.45 and 45.15 s −1 , respectively. Direct electron transfer properties of the nanostructured film result in 3 rd generation cholesterol biosensor. The bioelectrode exhibits high sensitivity (2.15 mAM −1 cm −2 ), a low K m app value (0.07 mM), good stability and high selectivity towards cholesterol.