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Comparative Analysis of Cellular Prion Detection by Mass‐Sensitive Immunosensors
Author(s) -
Poturnayova A.,
Snejdarkova M.,
Babelova L.,
KorriYoussoufi H.,
Hianik T.
Publication year - 2014
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400049
Subject(s) - quartz crystal microbalance , detection limit , dendrimer , chemistry , chromatography , antibody , matrix (chemical analysis) , biochemistry , biology , adsorption , immunology , organic chemistry
We performed a comparative study of the detection of human cellular prions (PrP C ) with a quartz crystal microbalance (QCM) immunosensor using various antibodies against PrP C . The sensing layer was formed by immobilized antibodies on a surface of polyamidoamine dendrimers (PAMAM) of fourth generation (G4) with attached protein A. The sensitivity of detection depended on the kind of antibody used and the best detection limit was 0.8 nM. PrP C detection in 10 times diluted blood plasma revealed more complicated behavior of motional resistance, which is evidence on viscosity contribution. The sensor recovery was 82.2 %, suggesting certain matrix effects on prion detection.