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Implications of FAD Electrode Reaction Kinetics for Electrocatalysis of NADH Oxidation and Development of NAD‐Dependent Enzyme Electrodes
Author(s) -
Emre Fatma Bilge,
Lopes Paula,
Ferapontova Elena E.
Publication year - 2014
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400048
Subject(s) - redox , nad+ kinase , dehydrogenase , chemistry , electrocatalyst , electrode , catalysis , kinetics , inorganic chemistry , enzyme , electrochemistry , combinatorial chemistry , biochemistry , quantum mechanics , physics
FAD entrapped into the polyethyleneimine (PEI) matrix underwent 2e − /2H + redox transformation, in contrast to the 1e − /1H + reaction observed with non‐stabilized FAD‐modified electrodes. Under conditions of the 2e − /2H + reaction, FAD/PEI‐modified electrodes catalytically oxidized NADH starting from −100 mV vs. Ag/AgCl. These electrodes may be used for construction of NAD‐dependent dehydrogenase electrodes for clinically important analytes such as glucose when coupled to the model enzyme glucose dehydrogenase. Latter allows detection of glucose starting from −100 mV within 0.05–5 mM concentration range. These results suggest the FAD stabilization by different matrices as a simple and promising approach to achieve its 2e − /2H + redox chemistry resulting in catalysis of NADH oxidation.