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Evaluation of Some Redox Mediators in the Design of Reagentless Amperometric Glucose Biosensor
Author(s) -
KausaiteMinkstimiene Asta,
Mazeiko Viktor,
Ramanaviciene Almira,
Oztekin Yasemin,
Osman Solak Ali,
Ramanavicius Arunas
Publication year - 2014
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400023
Subject(s) - biosensor , glucose oxidase , tetrathiafulvalene , amperometry , chemistry , glutaraldehyde , redox , ferrocyanide , potassium ferricyanide , tetracyanoquinodimethane , methylene blue , prussian blue , potassium ferrocyanide , adsorption , electrode , chromatography , inorganic chemistry , electrochemistry , organic chemistry , biochemistry , catalysis , molecule , photocatalysis
The influence of redox mediators (tetrathiafulvalene (TTF), tetrathiafulvalene‐tetracyanoquinodimethane complex (TTF‐TCNQ), phenazine methosulfate (PMS), 5,6‐diamino‐1,10‐phenonthroline (5,6‐DAP), potassium ferrocyanide (K4[Fe(CN)6]), methylene blue (MB) and toluidine blue (TB)) on the response of amperometric glucose biosensor was investigated in this research. Graphite rod electrode (GRE) modified by adsorbed mediator and glucose oxidase (GOx) cross‐linked with glutaraldehyde was served as working electrode. The highest amperometric signals were observed by using TTF and TTF‐TCNQ as mediators. The impact of amount of immobilized GOx, an optimal pH region for operation, stability and reproducibility of the analytic signal of designed biosensor using these mediators were investigated and discussed.