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Composite Material Based on Macroporous Polyaniline and Osmium Redox Complex for Biosensor Development
Author(s) -
Antiochia Riccarda,
Mazzei Franco,
Gorton Lo,
Leech Dónal,
Favero Gabriele
Publication year - 2014
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201400021
Subject(s) - polyaniline , glucose oxidase , biosensor , materials science , osmium , redox , polystyrene , conductive polymer , polymerization , electrochemistry , chemical engineering , polymer , nanotechnology , chemistry , electrode , organic chemistry , composite material , catalysis , ruthenium , engineering , metallurgy
Here the feasibility of layers based on the conducting polymer polyaniline (PANI) as component of glucose biosensors using glucose oxidase (GOx) as enzyme and [Os(bpy) 2 (4‐aminomethylpyridine)Cl]PF 6 (OsCmplx) as electrochemical mediator, is evaluated. Particularly, PANI was employed to obtain a nanostructured macroporous material (m‐PANI) around polystyrene nanoparticles taken as template and the mediator was co‐immobilized during the polymerizing procedure. The GOx biosensor based on OsCmplx modified m‐PANI provides a linear response to glucose concentration in the range 5 up to 65 mM with a sensitivity of 3.54 µA/mM/cm 2 (on a projected geometric area=0.07 cm 2 ), an LOD of 0.8 mM and a good precision (% RSD ≤7, n =5); the biosensor is stable showing a decrease of 10% to the value of the sensitivity after 15 days of use and of about 50% after 40 days.