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Point‐of‐Care Amperometric Testing of Diabetic Marker (HbA1c) Using Specific Electroactive Antibodies
Author(s) -
Chopra Adity,
Rawat Sanjay,
Bhalla Vijayender,
Suri C. Raman
Publication year - 2014
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201300646
Subject(s) - amperometry , glycated hemoglobin , immunoassay , chemistry , chromatography , point of care , hemoglobin , colloidal gold , antibody , electrochemistry , nanotechnology , electrode , nanoparticle , biochemistry , immunology , materials science , medicine , diabetes mellitus , pathology , endocrinology , type 2 diabetes
Specific immune detection of glycated hemoglobin is still a great challenge owing to the small epitopic difference between Hemoglobin (Hb) and HbA1c. We report a new electrochemical immunoassay format for point of care testing of HbA1c. A conducting self‐assembled monolayer of mercaptophenyl boronic acid (MPBA) was used as a capture layer for binding of glycated proteins and ferrocene tagged anti‐HbA1c antibody (FcAb) as a tracer molecule on a gold screen printed electrode. Validation of the new HbA1c assay was carried out using 6 clinical samples with known HPLC values and a correlation coefficient of 98 % was observed.