Premium
Highly Sensitive Electrochemiluminescence Detection of Mercury(II) Ions Based on DNA‐Linked Luminol‐Au NPs Superstructure
Author(s) -
Wan Jun,
Yin Guang,
Ma Xiuju,
Xing Ling,
Luo Xiliang
Publication year - 2014
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201300628
Subject(s) - luminol , electrochemiluminescence , detection limit , chemistry , mercury (programming language) , metal ions in aqueous solution , dna , thymine , ion , nanoparticle , linear range , nanotechnology , materials science , chromatography , organic chemistry , biochemistry , programming language , computer science
A novel highly sensitive electrochemiluminescence (ECL) detection protocol for mercury(II) ions was developed. Based on the strong and stable thyminethymine mismatches complexes coordination chemistry, mercury(II) ions can specifically bind to a designed DNA strand, leading to the release of the complimentary DNA strand. The released DNA strand was then captured by magnetic beads modified with specific DNA, and then through the formation of DNA‐linked luminol‐Au nanoparticles (NPs) superstructure, a specific ECL system for mercury(II) ions was developed. Using 3‐aminopropyl‐triethoxysilane as an effective enhancer, the ECL system can detect Hg 2+ ion within a linear range from 2.0×10 −10 mol L −1 to 2.0×10 −8 M, with a detection limit as low as 1.05×10 −10 M (3 σ ). Moreover, this ECL system is highly specific for Hg 2+ , without interference from other commonly coexisted metal ions, and it can be used for the analysis of real samples.