Premium
Poly‐ε‐Lysine Modified Nanocarbon Film Electrodes for LPS Detection
Author(s) -
Kato Dai,
Oda Atsumu,
Tanaka Mutsuo,
Iijima Seiichiro,
Kamata Tomoyuki,
Todokoro Masami,
Yoshimi Yasuo,
Niwa Osamu
Publication year - 2014
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201300542
Subject(s) - electrode , lysine , ferrocene , adsorption , electrochemistry , lipopolysaccharide , chemistry , ion , materials science , combinatorial chemistry , organic chemistry , biochemistry , amino acid , medicine , endocrinology
We developed an electrochemical system for detecting lipopolysaccharide (LPS) that uses an ultraflat nanocarbon film electrode modified with poly‐ε‐lysine with a high affinity to LPS. LPS was captured on the modified electrode, and then ferrocene labeled polymyxin B (FcPMB) was captured on the LPS adsorbed electrode via the LPS‐PMB affinity interaction. The adsorbed FcPMB provided an amplified response with Fe 2+ ions, and the current response was dependent on the amount of captured LPS ( LOD =2.0 ng/mL). This was due to the efficient accumulation of the obtained current for LPS and the very low noise made possible by the ultraflat surface.