Premium
A Label‐Free Electrochemical Aptasensor for Thrombin Using a Single‐Wall Carbon Nanotube (SWCNT) Casted Glassy Carbon Electrode (GCE)
Author(s) -
Park Kyungsoon,
Kwon Seong Jung,
Kwak Juhyoun
Publication year - 2014
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201300524
Subject(s) - electrochemistry , carbon nanotube , guanine , aptamer , electrode , glassy carbon , stacking , nanotube , overpotential , materials science , combinatorial chemistry , chemical engineering , chemistry , inorganic chemistry , nuclear chemistry , nanotechnology , nucleotide , organic chemistry , cyclic voltammetry , biochemistry , engineering , gene , biology , genetics
An electrochemical aptasensor with a thrombin binding aptamer (TBA) was developed using a single‐wall carbon nanotube (SWCNT) casted GCE. The TBA was immobilized on SWCNTs through π‐stacking without any special modification, resulting in helical wrapping to the surface. In the presence of thrombin, the TBA binds with thrombin and the TBA concentration on the SWCNT surface decreases. The remaining amount of TBA can be analyzed by an electrochemical method without any label, because the guanine bases of the nucleic acid are measurable by electrochemical methods. The electrochemical oxidation of guanine nucleotides was enhanced by electrocatalytic mediation using Ru(bpy) 3 2+ for higher sensitivity and reduction of the overpotential for electrochemical detection.