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Ultrasensitive Immunoassay Based on Amplified Inhibition of the Electrochemical Stripping Signal of Silver Nanocomposite by Silica Nanoprobe
Author(s) -
Yin Cuiying,
Lai Guosong,
Fu Li,
Zhang Haili,
Yu Aimin
Publication year - 2014
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201300507
Subject(s) - nanoprobe , detection limit , immunoassay , reproducibility , nanocomposite , stripping (fiber) , colloidal gold , materials science , linear range , electrochemistry , nanotechnology , chromatography , nanoparticle , chemistry , electrode , antibody , immunology , composite material , biology
A silver nanocomposite was one‐step synthesized in chitosan solution and used to prepare an immunosensor with the aid of gold nanoparticles (Au NPs) assembly. The Ag NPs at the immunosensor exhibited sensitive electrochemical stripping signal in KCl solution. After a sandwich immunoreaction, the current response of the immunosensor decreased due to the formation of antibody‐antigen immunocomplex on its surface, which was greatly amplified by the captured silica nanoprobes and thus enabled an ultrasensitive electrochemical immunoassay method. This method showed excellent analytical performance for human IgG measurement including wide linear range, low detection limit, cheap cost, satisfactory reproducibility and stability.