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A High Performance Electrochemical Biosensing Platform for Glucose Detection and IgE Aptasensing Based on Fe 3 O 4 /Reduced Graphene Oxide Nanocomposite
Author(s) -
Teymourian Hazhir,
Salimi Abdollah,
Firoozi Somayeh
Publication year - 2014
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201300496
Subject(s) - differential pulse voltammetry , graphene , dielectric spectroscopy , biosensor , glucose oxidase , nanocomposite , electrochemistry , nuclear chemistry , aptamer , cyclic voltammetry , oxide , chemistry , materials science , electrode , nanotechnology , organic chemistry , biology , genetics
A Fe 3 O 4 magnetic nanoparticles/reduced graphene oxide nanocomposite modified glassy carbon electrode (Fe 3 O 4 /r‐GO/GCE) was further expanded into the design of a high performance electrochemical biosensing platform. First, direct electrochemistry of glucose oxidase(GOx) immobilized onto Fe 3 O 4 /r‐GO/GCE and its biocatalytic activity toward glucose oxidation was investigated. Then, differential pulse voltammetry (DPV) was used to investigate the electrochemical oxidation behavior of four DNA free bases of guanine (G), adenine (A), thymine (T) and Cytosine (C) at Fe 3 O 4 /r‐GO/GCE. In addition, a label‐free electrochemical impedance spectroscopy (EIS) method of detection was developed for Immunoglobulin E (IgE) in which the binding of IgE‐specific aptamers immobilized on Fe 3 O 4 /r‐GO/GCE leads to impedance changes associated with IgE binding events.