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Electrochemiluminescence Biosensor Based on PEDOT‐PSS‐ Graphene Functionalized ITO Electrode
Author(s) -
Gao Ying,
Li Jing,
Yang Xiaodong,
Xiang Qian,
Wang Kunqi
Publication year - 2014
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201300470
Subject(s) - electrochemiluminescence , pedot:pss , polystyrene sulfonate , graphene , biosensor , materials science , electrode , sulfonate , reagent , nanocomposite , nanotechnology , chemical engineering , chemistry , organic chemistry , sodium , layer (electronics) , engineering , metallurgy
A novel and sensitive electrochemiluminescence (ECL) method for ethanol biosensor was developed by co‐immobilizing the enzyme and ECL reagent Ru(bpy) 3 2+ on the poly‐(3,4‐ethylene dioxythiophene) and polystyrene sulfonate functionalized graphene (PEDOT‐PSS‐G) nanocomposite film. Positively charged Ru(bpy) 3 2+ could be immobilized effectively on the electrode surface with the negatively charged PSS and graphene, which provided a stable ECL platform for further modification with the enzyme. Moreover, the introduction of PEDOT and graphene can be acted as a conducting pathway to accelerate the electron transfer due to the high conductivity. Such biosensor combined enzymatic selectivity with the amplification of PEDOT‐PSS‐G performed well with a wide linear range, high sensitivity and good stability. The sensing platform was successfully applied to determine the amounts of alcohol in real samples.