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Introduction of Ketamine as a G‐Quadruplex‐Binding Ligand Using Platinum Nanoparticle Modified Carbon Paste Electrode
Author(s) -
Bagheryan Zahra,
Raoof JahanBakhsh,
Ojani Reza,
HamidiAsl Ezat
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201300418
Subject(s) - differential pulse voltammetry , circular dichroism , intercalation (chemistry) , platinum nanoparticles , platinum , ligand (biochemistry) , carbon paste electrode , nanoparticle , chemistry , electrode , electrochemistry , cyclic voltammetry , analytical chemistry (journal) , nuclear chemistry , materials science , crystallography , nanotechnology , inorganic chemistry , chromatography , organic chemistry , biochemistry , receptor , catalysis
We designed an electrochemical platform by modifying a carbon paste electrode (CPE) with platinum nanoparticles to study the interaction between ketamine and the G‐quadruplex structure of human telomeric DNA (G 4 HTD). The drug ketamine (Kt) was used as the model ligand and its ability for stabilizing the G‐quadruplex structure was examined. The modified CPE (NPtCPE) was characterized by differential pulse voltammetry (DPV) and atomic force microscopy (AFM). The interaction of Kt with G 4 HTD was studied by DPV and the DPV current decreased with increasing Kt concentration. The results from UV‐vis and circular dichroism (CD) spectroscopy showed a prominent intercalation mode between G 4 HTD and Kt.