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A Ready‐to‐Use Electrochemical Kit Design for the Diagnosis of Single Nucleotide Polymorphisms
Author(s) -
Kayran Yasin Ugur,
OzkanAriksoysal Dilsat,
Tezcanli Burcin,
Kosova Buket,
Ozsoz Mehmet
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201300386
Subject(s) - single nucleotide polymorphism , detection limit , guanine , catechol , nucleotide , electrochemistry , polymorphism (computer science) , chemistry , microbiology and biotechnology , chromatography , combinatorial chemistry , gene , electrode , allele , biology , biochemistry , genotype
In this study, for the first time a model electrochemical kit was constructed for the detection of a functional polymorphism in catechol‐ O ‐methyl transferase (COMT) gene which is important for diagnosis of neuropsychiatric disorders as Alzheimer disease. The disposable pencil graphite electrode (PGE) is designed as a “kit” and the probe DNA covered PGE can detect single nucleotide polymorphisms (SNPs) from real samples based on the guanine oxidation signal even after 5 months of kit preparation (150 days durability).The detection limit ( S/N =3) of the biosensor was calculated as 1.18 pmol of synthetic target sequence and 6.09×10 5 molecules of real samples in 30 min detection time.