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Electrochemical Magnetic Immunosensors for the Determination of Ceruloplasmin
Author(s) -
Ojeda Irene,
MorenoGuzmán María,
GonzálezCortés Araceli,
YáñezSedeño Paloma,
Pingarrón José M.
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201300269
Subject(s) - ceruloplasmin , chemistry , conjugate , streptavidin , chromatography , immunoassay , electrochemistry , calibration curve , alkaline phosphatase , detection limit , electrode , biotin , antibody , biochemistry , enzyme , mathematical analysis , mathematics , immunology , biology
Electrochemical immunosensors for ceruloplasmin (Cp) are reported for the first time. Two configurations involving magnetic beads (MBs) functionalized with Protein A or Streptavidin for immobilization of Cp antibodies were compared, using competitive immunoassay with synthesized alkaline phosphatase‐Cp conjugate. Upon capturing MBs‐immunoconjugates onto screen‐printed carbon electrodes, quantification of Cp was accomplished by DPV measurement of 1‐naphthol generated after 1‐naphthylphosphate addition. Linear ranges of calibration curves and detection limits were 0.1–1000 µg/mL and 0.040 µg/mL (Protein A‐MBs), and 0.025–20 µg/mL and 0.018 µg/mL (Strept‐MBs). Good results were obtained in the determination of Cp in spiked human serum samples.