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1,4‐Michael Addition – The Analytical Way for Quantitative Sensing of Neurotransmitter at Bare Gold Electrode in Physiological Solution in the Presence of Interfering Biogenic Compounds
Author(s) -
Łuczak Teresa
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201300264
Subject(s) - chemistry , detection limit , morpholine , ascorbic acid , nucleophile , mass spectrometry , norepinephrine , michael reaction , chromatography , organic chemistry , dopamine , food science , neuroscience , biology , catalysis
Electrooxidation of norepinephrine in the presence a nucleophile was investigated on a bare gold electrode. Electrochemically produced norepinephrinequinone undergoes an attack by morpholine as nucleophile via 1,4‐Michael addition. The reaction products were identified by electrospray ionization mass spectrometry. The procedure used was suitable for quantitative norepinephrine determination in the concentration range from 1×10 −9 M to 8×10 −4 M with a detection limit of 8.7×10 −10 M in a samples containing an excess of ascorbic and uric acids. The proposed method is simple, green which means that it does not require the use of toxic compounds and solvents and thus is promising for detection of norepinephrine in physiological environment.