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Effective and Functional Surface Design for Biosensing Applications Based on a Novel Conducting Polymer and PMMA/Clay Nanocomposite
Author(s) -
Kesik Melis,
Kocer Ozgecan,
Kanik Fulya Ekiz,
Unlu Naime Akbasoglu,
Rende Eda,
AslanGurel Evren,
Rossi Rene M.,
Udum Yasemin Arslan,
Toppare Levent
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201300193
Subject(s) - biosensor , nanocomposite , glucose oxidase , materials science , surface modification , polymer , monomer , detection limit , chemical engineering , in situ polymerization , polymer chemistry , nanotechnology , polymerization , chemistry , composite material , chromatography , engineering
Abstract Surface functionalization plays a crucial role in the design of biosensors. For this purpose, a novel functional monomer, 6‐(4,7‐bis(2,3‐dihydrothieno[3,4‐ b ][1,4]dioxin‐5‐yl)‐2 H ‐benzo[ d ][1,2,3]triazol‐2‐yl)hexan‐1‐amine (BEDOA‐6), was designed and synthesized. Poly(BEDOA‐6) was utilized as an immobilization matrix for glucose oxidase biosensor construction. Moreover, polymethylmethacrylate (PMMA) layered silicate nanocomposites were prepared by in situ suspension polymerization. Conducting polymer surface was modified with PMMA/clay nanocomposite material and a glucose biosensor was developed. In addition, XPS and SEM were utilized to characterize the surface properties. The biosensor shows a wide linear range between 2.8 µM and 1.2 mM to glucose with a low detection limit of 1.99 µM. Finally, the biosensor was tested on serum samples containing actual human blood. The results were in well‐agreement with a reference method.