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G‐Quadruplex‐Linked Supersandwich DNA Structure for Electrochemical Amplified Detection of Thrombin
Author(s) -
Wang Guangfeng,
He Xiuping,
Zhu Yanhong,
Chen Ling,
Wang Lun,
Zhang Xiaojun
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201300184
Subject(s) - hemin , aptamer , g quadruplex , detection limit , dna , deoxyribozyme , combinatorial chemistry , thrombin , chemistry , conjugated system , electrochemistry , nanotechnology , materials science , biochemistry , microbiology and biotechnology , chromatography , electrode , biology , heme , organic chemistry , platelet , immunology , enzyme , polymer
Abstract In this paper, a novel strategy of electrochemical amplified detection of thrombin based on G‐quadruplex‐linked supersandwich structure was described. In the presence of K + and hemin, the original hairpin DNA sequence activated an autonomous cross‐opening process to build up hemin/G‐quadruplex structure and can hybridize to form supersandwich structure containing multiple signal labels. With the addition of thrombin, it conjugated with its aptamer, leading to a remarkably descended signal. The supersandwich‐amplified electrochemical sensor system was highly sensitive in the concentration range from 10 −6 to 10 −10 M with a detection limit of 10 pM and also demonstrated excellent selectivity. The amplifying supersandwich structure with multiple labels can be implemented as a versatile sensing platform for analyzing other DNA in the presence of the appropriate probe.