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Chemometric Strategies to Develop a Nanocomposite Electrode for Simultaneous Determination of Ascorbic Acid, Dopamine, and Uric Acid
Author(s) -
de Pieri Troiani Estela,
Rodrigues PereiraFilho Edenir,
Censi Faria Ronaldo
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201300166
Subject(s) - ascorbic acid , differential pulse voltammetry , nanocomposite , detection limit , uric acid , chemistry , nuclear chemistry , electrode , factorial experiment , carbon nanotube , electrochemistry , cyclic voltammetry , chromatography , materials science , analytical chemistry (journal) , nanotechnology , mathematics , biochemistry , statistics , food science
A simple and sensitive method for simultaneously measuring dopamine (DA), ascorbic acid (AA), and uric acid (UA) using a poly(1‐aminoanthracene) and carbon nanotubes nanocomposite electrode is presented. The experimental parameters for composite film synthesis as well as the variables related to simultaneous determination of DA, AA, and UA were optimized at the same time using fractional factorial and Doehlert designs. The use of carbon nanotubes and poly(1‐aminoanthracene) in association with a cathodic pretreatment led to three well‐defined oxidation peaks at potentials around −0.039, 0.180 and 0.351 V (vs. Ag/AgCl) for AA, DA, and UA, respectively. Using differential pulse voltammetry, calibration curves for AA, DA, and UA were obtained over the range of 0.16–3.12×10 −3 mol L −1 , 3.54–136×10 −6 mol L −1 , and 0.76–2.92×10 −3 mol L −1 , with detection limits of 3.95×10 −5 mol L −1 , 2.90×10 −7 mol L −1 , and 4.22×10 −5 mol L −1 , respectively. The proposed method was successfully applied to determine DA, AA, and UA in biological samples with good results.