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Electrochemical Aptasensor for the Determination of Ochratoxin A at the Au Electrode Modified with Ag Nanoparticles Decorated with Macrocyclic Ligand
Author(s) -
Evtugyn Gennady,
Porfireva Anna,
Sitdikov Rusal,
Evtugyn Vladimir,
Stoikov Ivan,
Antipin Igor,
Hianik Tibor
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201300164
Subject(s) - aptamer , ferricyanide , electrochemistry , chemistry , catechol , ochratoxin a , covalent bond , electrode , silver nanoparticle , nanoparticle , colloidal gold , combinatorial chemistry , ferrocene , inorganic chemistry , nanotechnology , materials science , organic chemistry , genetics , food science , mycotoxin , biology
An electrochemical aptasensor for ochratoxin A (OTA) detection has been developed on the base of a gold electrode covered with electropolymerized neutral red and silver nanoparticles obtained by chemical reduction with macrocyclic ligands bearing catechol fragments. Thiolated aptamers against OTA were covalently attached to silver nanoparticles via AgS bonding. The interaction with OTA induced the conformational switch of the aptamer, which caused increase of the charge transfer resistance measured by EIS in the presence of ferricyanide ions. The LOD achieved (0.05 nM) was comparable to other electrochemical aptasensors employing sophisticated assembling technique and enzyme amplification of the signal. The aptasensor was validated in spiked beer samples. The recovery of the OTA determination was found to be 66.3±14.1 % for light beer and 64.3±1.8 % for dark beer.