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Electrochemical Estrogen Receptor α based Biosensor for Label‐Free Detection of Estradiol
Author(s) -
Guo Zhenzhong,
Zine Nadia,
Balaguer Patrick,
Zhang Aidong,
Namour Philippe,
Lagarde Florence,
JaffrezicRenault Nicole
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201300163
Subject(s) - detection limit , biosensor , estrogen receptor , electrochemistry , linear range , chemistry , electrode , estrogen , selectivity , chromatography , combinatorial chemistry , medicine , endocrinology , biochemistry , biology , breast cancer , cancer , catalysis
We describe a new electrochemical biosensor based on estrogen receptor α (ER‐α) for label‐free detection of 17β‐estradiol, a model of endocrine‐disrupting compounds. ER‐α is coupled onto the gold electrode through its 6‐His tag and NTA‐copper complex. After interaction of estradiol with ER‐α, the biosensor presents a well‐defined peak at +500 mV due to estradiol oxidation (E17 peak). The linear range of detection is from 1 fM to 1 nM and the detection limit is 1 fM. Good selectivity was obtained for interfering substances at nanomolar level, for concentration of E17 up to 0.1 pM. The E17 was detected in hospital effluents.