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Electrochemical Detection of NADH, Cysteine, or Glutathione Using a Caffeic Acid Modified Glassy Carbon Electrode
Author(s) -
Lee P. T.,
Compton R. G.
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201300145
Subject(s) - cyclic voltammetry , electrode , caffeic acid , glassy carbon , cysteine , glutathione , electrochemistry , chemistry , biosensor , carbon fibers , detection limit , inorganic chemistry , nuclear chemistry , materials science , organic chemistry , chromatography , antioxidant , biochemistry , enzyme , composite material , composite number
Abstract A modified electrode was prepared using electrodeposition methods to immobilize caffeic acid (CAF) onto the surface of a glassy carbon electrode (GCE) to create a polymer suitable for biosensor development. The polymer film coverage of the surface bound species was further optimized using electrodeposition methods, thus increasing the surface coverage to ca. 10 −9 mol cm −2 . Using cyclic voltammetry, the modified carbon electrode was used to facilitate and observe the electrocatalytic oxidation of coenzymes such as NADH, cysteine, and glutathione at different concentrations. A calibration curve was determined in each case within the concentration range; 300 nM to 10 mM, with the limits of detection ( LOD ) of 246 µM, 99 µM, 2.2 µM for NADH, cysteine, and glutathione respectively.