Premium
Amperometric Detection of Histamine with a Pyrroloquinoline‐Quinone Modified Electrode
Author(s) -
Young Joshua A.,
Jiang Xiayu,
Kirchhoff Jon R.
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201300114
Subject(s) - pyrroloquinoline quinone , amperometry , polypyrrole , detection limit , electrode , linear range , chemistry , pyrrole , glassy carbon , chromatography , organic chemistry , electrochemistry , enzyme , cofactor , cyclic voltammetry
A modified glassy carbon (GC) electrode was developed for the amperometric detection of biogenic amines, particularly histamine. The electrode was modified with the co‐enzyme pyrroloquinoline quinone (PQQ) by entrapment during electropolymerziation of pyrrole to form polypyrrole (PPy). This method formed a thin film on the electrode surface possessing very good stability with a shelf‐life exceeding one month without loss of signal. Optimal conditions for the PQQ/PPy electrode were determined and a linear response was found for histamine in phosphate buffer (pH 6) at +550 mV from 40 to 170 mg L −1 with a limit of detection ( S/N ≥3) of 38 mg L −1 . The practical linear range offered by this method suggests ideal use for spoilage detection in fermented foods.