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Pencil Lead Electrode Modified with Hemoglobin Film as a Novel Biosensor for Nitrite Determination
Author(s) -
Majidi Mir Reza,
Saadatirad Afsaneh,
Alipour Esmaeel
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201300082
Subject(s) - nitrite , chronoamperometry , detection limit , differential pulse voltammetry , chemistry , cyclic voltammetry , electrochemistry , electrode , voltammetry , linear range , analytical chemistry (journal) , amperometry , inorganic chemistry , chromatography , organic chemistry , nitrate
In the present paper, the electrochemical reduction of nitrite at a hemoglobin modified pencil lead electrode (Hb/PLE) is described. The electrochemical properties of nitrite were studied by cyclic voltammetry and chronoamperometry. Results showed that the hemoglobin film has an excellent electrochemical activity towards the reduction of nitrite. By using voltammetric and chronoamperometric methods, α , n α and n were calculated. Then the ability of the electrode for nitrite determination was investigated using differential pulse voltammetry. The electrocatalytic reduction peak currents were found to be linear with the nitrite concentration in the range from 10 to 220 µM with a detection limit of 5 µM. The relative standard deviation is 2 % for 3 successive determinations of a 100 µM nitrite solution. This modified electrode was successfully used for the detection of low amounts of NO 2 − in spinach sample and a spiked sample of tap water.