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Investigation of Protein Binding With All Solid‐State Ion‐Selective Electrodes
Author(s) -
Prabhu Alok,
Bobacka Johan,
Ivaska Ari,
Levon Kalle
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201300071
Subject(s) - potentiometric titration , chemistry , ionophore , ion , bovine serum albumin , electrode , ion selective electrode , chloride , lysozyme , analytical chemistry (journal) , inorganic chemistry , chromatography , selectivity , organic chemistry , biochemistry , membrane , catalysis
Abstract A potentiometric sensor for studying charge based adsorption of proteins was created using a single‐piece polyaniline‐PVC ion‐selective electrode (ISE). Three different ISEs, two for Na + and one for Cl − ion determination, were studied. The Na + ‐ISEs consisted of a neutral calixarene‐based ionophore and one with a charged carrier dinonylnapthalenesulfonic acid (DNNSA) whereas for the Cl − ISE, an anion exchanger tridodecylmethylammonium chloride (TDDMA + Cl − ), was used. The Na + ISE with DNNSA as the charged carrier was successfully able to discriminate the binding of two different proteins (bovine serum albumin and lysozyme) based on their intrinsic charge.