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Molecularly Imprinted Polypyrrole for DNA Determination
Author(s) -
Ratautaite Vilma,
Topkaya Seda Nur,
Mikoliunaite Lina,
Ozsoz Mehmet,
Oztekin Yasemin,
Ramanaviciene Almira,
Ramanavicius Arunas
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201300063
Subject(s) - polypyrrole , differential pulse voltammetry , electrode , cyclic voltammetry , molecularly imprinted polymer , electrochemistry , materials science , dna , layer (electronics) , voltammetry , chemistry , analytical chemistry (journal) , nanotechnology , chromatography , selectivity , organic chemistry , biochemistry , catalysis
In this study graphite electrodes modified by a thin DNA‐imprinted polypyrrole layer, which was able to bind specific target‐DNA, are reported. For this aim, electrochemical synthesis of polypyrrole was performed on a pencil graphite electrode by cyclic voltammetry (CV) or by potential pulse sequences (PPS). The modified electrode surface was used for electrochemical determination of target‐DNA by differential pulse voltammetry. According to our best knowledge this is a first report on the application of DNA‐imprinted polymer for the determination of target‐DNA. The results showed that the molecularly imprinted polypyrrole (MIPPy) layer that formed on the carbon electrode surface was sensitive for target‐DNA, while the nonimprinted polypyrrole layer was not sensitive to the same target‐DNA. Comparison of electrodes modified using PPS and CV techniques is presented.