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Direct Electrocatalytic Oxidation and Simultaneous Determination of 5‐Methylcytosine and Cytosine at Electrochemically Reduced Graphene Modified Glassy Carbon Electrode
Author(s) -
Zheng Xiaoxia,
Wang Lishi
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201300040
Subject(s) - graphene , differential pulse voltammetry , glassy carbon , 5 methylcytosine , oxide , chemistry , electrode , voltammetry , electrocatalyst , carbon fibers , cytosine , inorganic chemistry , cyclic voltammetry , electrochemistry , analytical chemistry (journal) , nuclear chemistry , materials science , dna methylation , nanotechnology , chromatography , organic chemistry , dna , composite number , biochemistry , gene expression , composite material , gene
Direct electrocatalytic oxidation and simultaneous determination of 5‐methylcytosine (5‐mC) and cytosine(C) were realized in alkaline solutions by differential pulse voltammetry (DPV) based on an electrochemically reduced graphene oxide (er‐GO) modified glassy carbon electrode (er‐GO/GCE). The as‐prepared er‐GO/GCE exhibited good electrocatalytic activity towards the oxidation of 5‐mC and C. Under optimum conditions, the er‐GO/GCE was applied to the simultaneous determination of 5‐mC and C with a significantly improved peak potential resolution (about 150 mV), and a linear relationship can be obtained in the range of 6–200.0 µmol/L and 8–250.0 µmol/L, respectively. In addition, the proposed method was further successfully applied to the analysis of methylation status in short CpG oligonucleotides with satisfactory results.