Premium
Bilirubin Oxidase‐Based Nanobiocathode Working in Serum‐Mimic Buffer for Implantable Biofuel Cell
Author(s) -
GutiérrezSánchez Cristina,
Pita Marcos,
Toscano Miguel D.,
De Lacey Antonio L.
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201200668
Subject(s) - bilirubin oxidase , buffer (optical fiber) , chemistry , bilirubin , oxidase test , buffer solution , chromatography , biochemistry , enzyme , biology , telecommunications , computer science , endocrinology
Low‐density graphite electrodes have been covalently modified with gold nanoparticles and used as scaffold for the oriented immobilization of Myrothecium verrucaria bilirubin oxidase. The developed nanobioelectrodes have been tested as a biocathode in a human‐serum surrogate buffer that mimics physiological conditions. The nanostructured bioelectrode offered a −140 µA cm −2 current density under serum‐mimic conditions at 0.2 V, and was operational during at least 6 days.