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Disposable Horseradish Peroxidase Biosensors for the Selective Determination of Tyramine
Author(s) -
CalvoPérez Ana,
DomínguezRenedo Olga,
AlonsoLomillo M Asunción,
ArcosMartínez M Julia
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201200636
Subject(s) - horseradish peroxidase , biosensor , chemistry , tyramine , repeatability , chromatography , reproducibility , biochemistry , enzyme
This work reports the development of horseradish peroxidase based biosensors using screen‐printed carbon electrodes for the determination of tyramine (tyr). A novel procedure based on the insertion of the enzyme in the screen‐printing process (SPC HRP Es) has been compared with the cross‐linked immobilization into the carbon working electrode (HRP/SPCEs). Both biosensors were characterized obtaining good capability of detection (2.1±0.2 and 0.2±0.01 µM for SPC HRP Es and HRP/SPCEs, respectively). The reproducibility was 3.4 % and 6.8 % for SPC HRP Es and HRP/SPCEs, respectively. The repeatability was 2.2 % and 7.1 % for SPC HRP Es and HRP/SPCEs, respectively. The specificity towards different biogenic amines was analyzed. The developed biosensors were applied to the determination of tyr content in cheese samples.