Premium
The Enhanced Enzymolysis Resistance of Surface‐Immobilized DNA Caused by Hybridizing with Morpholino
Author(s) -
Cao Lei,
Zhao Yun,
Ji LiNa,
Zhang Jing,
Wang Kang,
Xia XingHua
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201200612
Subject(s) - dna , steric effects , chemistry , cyclic voltammetry , biochemistry , stereochemistry , electrochemistry , electrode
The anti‐enzymolysis capability of surface attached DNA, morpholino (MO) single strands, and DNA‐DNA, MO‐DNA double strands are studied using cyclic voltammetry. Results show that MO, the neutral analog of DNA, bears strong anti‐enzymolysis capability against DNase I and can protect its complementary DNA from being decomposed. Only 15 % of the DNA in the MO‐DNA double strands is decomposed within 20 min, which is much lower than that for DNA‐DNA double strands (100 % in 400 s). Fluorescence polarization measurements suggest that the steric hindrance of MO is the main cause of the improved anti‐enzymolysis ability of DNA in MO‐DNA double strands.