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Label‐Free Analysis of Thrombin or Hg 2+ Ions by Nucleic Acid‐Functionalized Graphene Oxide Matrices Assembled on Field‐Effect Transistors
Author(s) -
Sharon Etery,
Liu Xiaoqing,
Freeman Ronit,
Yehezkeli Omer,
Willner Itamar
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201200581
Subject(s) - aptamer , graphene , nucleic acid , yield (engineering) , oxide , ion , desorption , thrombin , chemistry , materials science , nanotechnology , analytical chemistry (journal) , biochemistry , organic chemistry , adsorption , genetics , platelet , immunology , metallurgy , biology
Graphene oxide (GO) is deposited onto the Al 2 O 3 gates of field‐effect transistor devices to yield a functional matrix for the sensing of thrombin or Hg 2+ ions. The deposition of an aptamer subunit against thrombin or a tailored nucleic acid with appropriate T mismatches on the GO, yield active surfaces for the analysis of thrombin or Hg 2+ ions. The desorption of the aptamer‐subunit from GO, through the formation of the aptamer‐thrombin complex, or the desorption of the T–Hg 2+ –T‐bridged duplex DNA alter the gate potential, thus providing a signal for the sensing events.