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Immunosensor Based on Electrogenerated Chemiluminescence Using Ru(bpy) 3 2+ ‐Doped Silica Nanoparticles and Calix[4]crown‐5 Self‐Assembled Monolayers
Author(s) -
Kim YangRae,
Seo HyoJu,
Oh JeongWook,
Lim Hyunchang,
Kim Tae Hyun,
Kim Hasuck
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201200548
Subject(s) - chemiluminescence , monolayer , detection limit , chemistry , microemulsion , nanoparticle , electrode , electrochemistry , colloidal gold , nuclear chemistry , chromatography , materials science , nanotechnology , pulmonary surfactant , biochemistry
An electrogenerated chemiluminescence (ECL)‐based immunosensor for the detection of immunoglobulin G (IgG) has been fabricated using Ru(bpy) 3 2+ ‐doped silica nanoparticles and calix[4]crown‐5 self‐assembled monolayers (SAMs). Ru(bpy) 3 2+ ‐doped silica nanoparticles are prepared by the water‐in‐oil (W/O) microemulsion method. ProLinker B, a commercially available thiolated calix[4]crown‐5 derivative, is utilized for the immobilization of anti‐immunoglobulin G (Anti‐IgG) on a gold electrode. The concentration of IgG is measured using a sandwich‐type ECL immunosensor based on the proposed immunosensor. The ECL intensity is linearly proportional to the IgG concentration over the concentration range 5–30 µg mL −1 . The detection limit of IgG is 1.5 µg mL −1 .