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Redox Active DipyrrometheneCu(II) Monolayer for Oriented Immobilization of His‐Tagged RAGE Domains – the Base of Electrochemical Biosensor for Determination of Aβ 16–23′
Author(s) -
Jargiło Agata,
Grabowska Iwona,
Radecka Hanna,
Sulima Magdalena,
Marszałek Ilona,
WysłouchCieszyńska Aleksandra,
Dehaen Wim,
Radecki Jerzy
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201200537
Subject(s) - biosensor , cyclic voltammetry , redox , dielectric spectroscopy , monolayer , chemistry , electrochemistry , electrode , voltammetry , analytical chemistry (journal) , inorganic chemistry , chromatography , biochemistry
Abstract The new system which consists of the thiol derivative of dipyrromethene–Cu(II) complex created on the surface of a gold electrode was applied for the first time for oriented immobilization of selected His‐tagged domains of a receptor for advanced glycation endproducts (RAGE). Cyclic voltammetry (CV), Osteryoung square‐wave voltammetry (OSWV), electrochemical impedance spectroscopy (EIS) and atomic force microscopy (AFM) were used for characterization of the redox active sensing layer. The biosensor proposed was applied for determination of Aβ 16–23′ peptide. In its presence, a decrease of the maximum Cu(II) redox current was observed. These values correlated linearly with the Aβ 16–23′ concentration in the range 0.001–1.000 µM. The presence of diluted human plasma has no influence on the sensor responses.