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A Comparative Study of Biocathodes Based on Multiwall Carbon Nanotube Buckypapers Modified with Three Different Multicopper Oxidases
Author(s) -
Pankratov Dmitry V.,
Zeifman Yulia S.,
Morozova Olga V.,
Shumakovich Galina P.,
Vasil'eva Irina S.,
Shleev Sergey,
Popov Vladimir O.,
Yaropolov Alexander I.
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201200516
Subject(s) - bilirubin oxidase , laccase , buckypaper , carbon nanotube , multicopper oxidase , chemistry , nanotechnology , fabrication , electrode , capacitance , materials science , chemical engineering , enzyme , organic chemistry , medicine , alternative medicine , pathology , engineering
14 Single‐ and multi‐walled carbon nanotubes from different sources were characterized in detail, and the characteristics obtained were carefully analyzed. The carbon material with the highest capacitance, and also other superior properties (“Taunit‐M” from “NanoTechCenter”, Russia), was chosen for further modification and fabrication of buckypaper based electrodes. These electrodes were biomodified with plant and fungal laccases, as well as fungal bilirubin oxidase. The designed biocathodes were investigated in simple buffers and also in a complex physiological fluid (human serum). Biocathodes based on immobilized fungal laccase were bioelectrocatalytically inactive in chloride containing media at neutral pH. In spite of the quite high current densities realized using biodevices based on plant laccase and fungal bilirubin oxidase, the limited thermal stability of the enzymes renders the biocathodes inadequate for practical applications in implanted situations.