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Amperometric Magnetoimmunosensors for Direct Determination of D‐Dimer in Human Serum
Author(s) -
Gamella María,
Campuzano Susana,
Conzuelo Felipe,
Reviejo A. Julio,
Pingarrón José M.
Publication year - 2012
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201200503
Subject(s) - amperometry , hydroquinone , dimer , electrode , detection limit , chemistry , electrochemistry , materials science , chromatography , organic chemistry
Two different D‐dimer disposable amperometric immunosensing designs based on indirect competitive or sandwich formats and the use of carboxylic acid‐modified magnetic beads (COOH‐MBs) and screen‐printed carbon electrodes (SPCEs) have been developed and compared. In both approaches, the resulting modified MBs were magnetically captured on the surface of a SPCE which was used as the transducer for the electrochemical detection at −0.20 V upon addition of H 2 O 2 , and hydroquinone (HQ). Both configurations exhibited linear ranges of clinical usefulness and detection limits quite below the clinical threshold (0.5 µg mL −1 D‐dimer). The sandwich configuration has been successfully tested with serum samples.