MWCNT Modified Glassy Carbon Electrode: Probing Furazolidone‐DNA Interactions and DNA Determination

The interactions of furazolidone (Fu) with double‐stranded calf thymus DNA (dsDNA) on the multiwalled carbon nanotubes modified glassy carbon electrode (MWCNT/GCE) have been studied by voltammetry and UV‐vis spectroscopy. The binding constants of Fu with DNA and the stoichiometric coefficient according to the Hill’s model have been determined. A detailed analysis of cyclic voltammograms gave fundamental electrochemical parameters including the electroactive surface coverage ( Γ ), the transfer coefficient ( α ), and the heterogeneous rate constant ( k s ). Two linear calibration curves were obtained for DNA detection in the concentration ranges of 40–100 µ L −1 and 100–1000 µg L −1 with a detection limit of 25 µg L −1 .