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MWCNT Modified Glassy Carbon Electrode: Probing Furazolidone‐DNA Interactions and DNA Determination
Author(s) -
Fotouhi Lida,
Bahmani Farzaneh
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201200495
Subject(s) - detection limit , cyclic voltammetry , electrode , analytical chemistry (journal) , stoichiometry , binding constant , electrochemistry , glassy carbon , calibration curve , voltammetry , carbon nanotube , materials science , dna , furazolidone , chemistry , nanotechnology , chromatography , binding site , biochemistry , antibiotics
The interactions of furazolidone (Fu) with double‐stranded calf thymus DNA (dsDNA) on the multiwalled carbon nanotubes modified glassy carbon electrode (MWCNT/GCE) have been studied by voltammetry and UV‐vis spectroscopy. The binding constants of Fu with DNA and the stoichiometric coefficient according to the Hill’s model have been determined. A detailed analysis of cyclic voltammograms gave fundamental electrochemical parameters including the electroactive surface coverage ( Γ ), the transfer coefficient ( α ), and the heterogeneous rate constant ( k s ). Two linear calibration curves were obtained for DNA detection in the concentration ranges of 40–100 µ L −1 and 100–1000 µg L −1 with a detection limit of 25 µg L −1 .