Premium
Application of Different Zeolites for Improvement of the Characteristics of a pH‐FET Biosensor Based on Immobilized Urease
Author(s) -
Soldatkin O. O.,
Kucherenko I. S.,
Shelyakina M. K.,
Soy E.,
Kirdeciler K.,
Öztürk S.,
JaffrezicRenault N.,
Akata B.,
Dzyadevych S. V.,
Soldatkin A. P.
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201200475
Subject(s) - urease , biosensor , urea , chemistry , zeolite , membrane , chromatography , bovine serum albumin , nuclear chemistry , inorganic chemistry , biochemistry , catalysis
Different modifications of the zeolites Na + ‐Beta and LTA were applied for improving the working characteristics of a urea biosensor. The bioselective membrane of the biosensor was based on urease and different zeolites co‐immobilized with bovine serum albumin on the surface of a pH‐FET. It was shown that the biosensors modified with the zeolites H + ‐Beta30 and H + ‐Beta50 are characterized by increased sensitivity to urea. The influence of the zeolite concentration on the sensitivity of the biosensors was studied. The optimal concentration of the zeolites H + ‐Beta30 and H + ‐Beta50 in the bioselective membrane was 15 %. Different variants of co‐immobilization of urease and zeolite H + ‐Beta30 were studied and the optimal method was selected. Thus, a general conclusion is that the urea biosensor sensitivity can be improved using zeolite H + ‐Beta30 for urease immobilization in the bioselective membrane.