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Molecular Imprint for Electrochemical Detection of Streptomycin Residues Using Enzyme Signal Amplification
Author(s) -
Que Xiaohua,
Liu Bingqian,
Fu Libing,
Zhuang Junyang,
Chen Guonan,
Tang Dianping
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201200468
Subject(s) - detection limit , differential pulse voltammetry , chemistry , molecularly imprinted polymer , glucose oxidase , hydrogen peroxide , chromatography , aniline , nuclear chemistry , enzyme , electrochemistry , catalysis , cyclic voltammetry , biochemistry , selectivity , electrode , organic chemistry
We have designed a new molecularly imprinted co‐polymer (MIP) for the sensitive detection of streptomycin (STR) in food using enzymes as signal amplification. The MIP was fabricated via co‐polymerization of aniline and o ‐phenylenediamine on gold substrate in the presence of STR as template. The assay is based on competitive binding of free STR and glucose oxidase‐labeled STR (GOx‐STR) to the imprinters on the MIP. On addition of glucose, hydrogen peroxide is formed that is detected by differential pulse voltammetry. Under optimal conditions, the decrease of the catalytic current is proportional to the STR concentration in the range from 0.01 to 10 ng mL −1 , with a detection limit ( LOD ) of 7.0 pg mL −1 STR (at 3 s B ). Intra‐ and inter‐assay coefficients of variation (CVs) are<10.5 %. The system was further validated and evaluated with STR‐spiked samples including honey and milk, and the recovery was between 82 and 124.2 %.