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Aptamer‐Assisted Gold Nanoparticles/PEDOT Platform for Ultrasensitive Detection of LPS
Author(s) -
Su Wenqiong,
Cho MiSuk,
Nam JaeDo,
Choe WooSeok,
Lee Youngkwan
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201200453
Subject(s) - dielectric spectroscopy , aptamer , cyclic voltammetry , colloidal gold , monolayer , pedot:pss , nanoparticle , materials science , nanotechnology , scanning electron microscope , conductive polymer , electrochemistry , electron transfer , chemical engineering , polymer , chemistry , photochemistry , electrode , layer (electronics) , composite material , biology , engineering , genetics
Abstract Neatly arranged gold nanoparticles (AuNPs) were directly electrodeposited on an electrochemically polymerized self‐assembled monolayer (SAM) of thiol‐functionalized 3,4‐ethylenedioxythiophene (EDOT) derivative, EDTMSHA. A thiolated single‐stranded DNA (ssDNA) aptamer with high specificity to LPS was immobilized on the AuNPs/conducting polymer composite film, serving as sensing platform for LPS detection. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), scanning electron microscope (SEM), and atomic force microscopy (AFM) were utilized to characterize the modification and detection processes. The electron transfer resistance was found to have a linear relationship with LPS concentration from 0.1 pg/mL to 1 ng/mL.