Premium
Oxygen Plasma Treatment of Platinized Ultramicroelectrodes Increases Sensitivity for Hydrogen Peroxide Detection on Mitochondria
Author(s) -
BenAmor Salem,
Devin Anne,
Rigoulet Michel,
Sojic Neso,
Arbault Stéphane
Publication year - 2013
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.201200409
Subject(s) - chemistry , chronoamperometry , hydrogen peroxide , oxygen , ultramicroelectrode , cyclic voltammetry , inorganic chemistry , analytical chemistry (journal) , electrochemistry , chromatography , electrode , biochemistry , organic chemistry
Hydrogen peroxide is a major bio‐active molecule produced by mitochondria during aerobic metabolism. In order to monitor that production, platinized ultramicroelectrodes with high surface‐area were used. Their sensitivity was significantly improved following an oxygen plasma treatment. Cyclic voltammetry and chronoamperometry analyses of ferricyanide reduction and hydrogen peroxide oxidation showed that ultramicroelectrodes’ capacitive current was decreased 3‐fold, while faradaic currents remained constant. Then, plasma‐treated ultramicroelectrodes allowed the chronoamperometric detection of 100 nM H 2 O 2 injections. This eventually enabled monitoring the mitochondria release of H 2 O 2 following injection of substrates and a respiratory inhibitor; a flux of few hundreds picomoles H 2 O 2 /mg protein/min was detected.